The aim of the study was to establish the association of genetic variants of the ARNTL1 gene with the onset of myocardial infarction.

Study design. Case-control study

Key words: ARNTL1 gene; circadian rhythm; myocardial infarction

Participants and Methods

The research is conducted on a total of 400 participants. In the first group there were 200 patients with myocardial infarction type 1 hospitalized at the University Hospital Osijek. The second group consisted of 200 healthy controls recruited by a family physician. The goal of the study was to determine the association between single nucleotide polymorphisms of ARNTL1 gene and myocardial infarction. All participants were genotyped for three single nucleotide polymorphisms in the ARNTL1 gene (rs3789327, rs4757144, rs12363415).


There was no statistically significant difference between polymorphisms of ARNTL1 gene in patients and controls. Research showed that there is no direct link between infarction and single nucleotide polymorphisms in the ARNTL1 gene, but rs3789327 and rs12363415 polymorphisms are associated with cardiovascular risk factors. Diabetes mellitus type 2 was associated with rs12363415 polymorphism, while a history of previous cardiovascular diseases was related to rs3789327 polymorphism. Type 2 diabetes mellitus is a significant risk factor because it has a major role in the development of atherosclerosis and coronary artery disease.


The results show that there is no association between SNP’s of ARNTL1 gene and myocardial infarction. However, association of SNPs and risk factors for the development of the infarction was found. Based on this, polymorphisms in the ARNTL1 gene has a certain role in the emergence of myocardial infarction.


Corresponding author:
Anton Šarčević, MD
Sveti Duh University Hospital, Zagreb, Croatia
KB Sveti Duh, Sveti Duh 64, 10 000 Zagreb
e-mail: asarcevic1201@gmail.com

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