To discuss the functional status-based personalized nursing in clinical care for acute leukemia. 175 patients with acute leukemia were divided into the conventional care group (patients within the group received routine care during chemotherapy for acute leukemia) and the observation group (patients within the group received functional status-based personalized care on the foundation of routine care) according to the method of random number table, and the mental status, quality of life, physical function status and complications during chemotherapy were compared between groups. Compared to the conventional care group following the intervention, the scores of self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were notably decreased in the observation group following the intervention. The scores of quality of life (QOL) and Karl Fischer Physical Functional Status Scale (KPS) were conspicuously increased, and the differences indicate statistical significance (p < 0.05); Compared with the conventional care group, the incidence of infection infectious fever, bleeding and ectopic liver function during chemotherapy in the study group were evidently reduced. The differences are statistically significant (p < 0.05). The functional status-based personalized nursing can effectively improve the mental status and quality of life, promote the physical function status, and effectively decrease the complications during chemotherapy in patients with acute leukemia, which is a safe and effective nursing model.