Article Data

  • Views 1425
  • Dowloads 146

Original Research

Open Access

Cord blood interleukin-8 levels correlate with airway flow limitation at eight years of age in ex-very low birth weight infants

  • IRENA ŠTUCIN GANTAR1
  • MARINA PRAPROTNIK2
  • JANEZ BABNIK1
  • BRANKA WRABER3
  • LILIJANA KORNHAUSER CERAR1

1Division of Perinatology, Department of Obstetrics and Gynecology University Medical Center Ljubljana

2Department of Pulmonology University Children`s Hospital, University Medical Centre Ljubljana

3Institute of Microbiology and Immunology, Medical Faculty Ljubljana, University of Ljubljana

DOI: 10.22514/SV82.102013.5 Vol.8,Issue 2,October 2013 pp.30-36

Published: 17 October 2013

*Corresponding Author(s): IRENA ŠTUCIN GANTAR E-mail: irena@gantar.com

Abstract

Background. Exposure to prenatal inflammation increases the risk for development of bronchopulmonary dysplasia. Aim. To evaluate the correlation between cord blood and gastric aspirate levels of interleukine-6 (IL-6) and interleukine-8 (IL-8) in preterm infants, and lung function at the age of 8 years.

Methods. Between 2000-2002 we recruited 129 infants of gestational age < 30 wks. The concentration of IL-6 and IL-8 were measured in gastric aspirate and cord blood. At the age of 8 years, 30 ex-preterm infants, with mean gestational age of 27 wks and mean birth weight of 955 g, returned for pulmonary function measurement. To exclude major bias, a comparison between the study group and non-responder group was done and showed no statistically significant difference with respect to perinatal characteristics, ventilation days, bronchopulmonary dysplasia and cytokine concentration. 

Results. Pulmonary function test measurments in children born preterm were lower than in their term pairs. However, only the difference in forced mid-expiratory flow (FEF25-75%) was statisticaly significant. The concentration of IL-6 and IL-8 in cord blood and in gastric aspirate inversely correlated to all parameters of lung fuction at the age of 8 years, however only correlations between the concentration of IL-8 in cord blood and forced expired volume in one second/forced vital capacity (FEV1/FVC) (r = -0.38, p = 0.04) and FEF 25%-75% (r = -0.44, p = 0.02) were statistically significant. 

Conclusion. These results show a negative correlation between the concentration of IL-8 in cord blood and FEF25%-75% and FEV1/FVC, which suggests the important role of IL-8 in early airway remodeling. 

Keywords

IL-8, lung function, pre-term infant

Cite and Share

IRENA ŠTUCIN GANTAR,MARINA PRAPROTNIK,JANEZ BABNIK,BRANKA WRABER,LILIJANA KORNHAUSER CERAR. Cord blood interleukin-8 levels correlate with airway flow limitation at eight years of age in ex-very low birth weight infants. Signa Vitae. 2013. 8(2);30-36.

References

1. Watterberg KL, Demers LM, Scott SM, Murphy S. Chorioamnionitis and early lung inflammation in infants in whom bronchopulmonary dysplasia develops. Pediatrics 1996;97:210􀀁5.

2. Hitti J, Krohn MA, Patton DL, Tarczy-Hornoch P, Hillier SL, Cassen EM, et al. Amniotic fluid tumor necrosis factor-alpha and the risk of respiratory distress syndrome among preterm infants. Am J Obstet Gynecol 1997;177:50􀀁6.

3. Ogunyemi D, Murillo M, Jackson U, Hunter N, Alperson B. The relationship between placental histopathology findings and perinatal outco-me in preterm infants. J Matern Fetal Neonatal Med 2003;13:102􀀁9.

4. Matsuda T, Nakajima T, Hattori S, Hanatani K, Fukazawa Y, Kobayashi K, et al. Necrotizing funisitis: clinical significance and association with chronic lung disease in premature infants. Am J Obstet Gynecol 1997;177:1402􀀁7.

5. Yoon BH, Romero R, Kim KS, Park JS, Ki SH, Kim BI, et al. A systemic fetal inflammatory response and the development of bronchopul-monary dysplasia. Am J Obstet Gynecol 1999;181:773-9.

6. Mittendorf R, Covert R, Montag AG, elMasri W, Muraskas J, Lee KS, et al. Special relationships between fetal inflammatory response syndrome and bronchopulmonary dysplasia in neonates. J Perinat Med 2005;33:428􀀁34.

7. Redline RW, Wilson-Costello D, Hack M. Placental and other perinatal risk factors for chronic lung disease in very low birth weight infants. Pediatr Res 2002;52:713􀀁9.

8. Kent A, Dahlstrom JE. Chorioamnionitis/funisitis and the development of bronchopulmonary dysplasia. J Paediatr Child Health 2004;40:356􀀁9.

9. Jobe AH. Antenatal factors and the development of bronchopulmonary dysplasia. Semin Neonatol 2003;8:9􀀁17.

10. Van Marter LJ, Dammann O, Allred EN, Leviton A, Pagano M, Moore M, et al. Chorioamnionitis, mecanical ventilation, and postnatal sepsis as modulators of chronic lung disease in preterm infants. J Pediatr 2002;140:171􀀁6.

11. Stucin Gantar I, Babnik J, Kornhauser Cerar L, Sinkovec J, Wraber B. Prenatal and postnatal risk factors for developing bronchopulmonary dysplasia. Signa Vitae 2011;6:46-51.

12. Been JV, Zimmermann LJ. Histological chorioamnionitis and respiratory outcome in preterm infants. Arch Dis Child Fetal Neonatal Ed 2009;94:18􀀁25.

13. Mu SC, Lin CH, Chen YL, Ma HJ, Lee JS, Lin MI, et al. Impact on neonatal outcome and anthropometric growth in very low birth weight infants with histological chorioamnionitis. J Formos Med Assoc 2008;107:304􀀁10.

14. Kilbride WH, Gelatt MC, Sabath RJ. Pulmonary function and exercise capacity for ELBW survivors in preadolescence: effect of neonatal chronic lung disease. J Pediatr 2003; 147:488􀀁93.

15. Narang I, Rosenthal M, Cremonesini D, Silverman M, Bush A. Longitudinal Evaluation of Airway Function 21 Years after Preterm Birth. Am J Respir Crit Care Med 2008;178: 74􀀁80.

16. Watterberg KL, Shaffer ML, Mishefske MJ, Leach CL, Mammel MC, Couser RJ, et al. Growth and neurodevelopmental outcomes after early low-dose hydrocortisone treatment in extremely low birth weight infants. Pediatrics 2007;120:40–8.

17. Kumar R, Yu Y, Story RE, Pongracic JA, Gupta R, Pearson C, et al. Prematurity, chorioamnionitis, and the development of recurrent whe-ezing: a prospective birth cohort study. J Allergy Clin Immunol 2008;121:878–84.

18. Shennan AT, Dunn MS, Ohlsson A, Lennox K, Hoskins EM. Abnormal pulmonary outcomes in premature infants: prediction from oxygen requirement in the neonatal period. Pediatrics 1988;82:527􀀁32

19. Dargaville PA, South M, Vervaart P, McDougall PN. Validity of markers of dilution in small volume lung lavage. Am J Respir Crit Care Med 1999;160:778􀀁84.

20. ATS/ERS Recommendations for standardized procedures for the online and offline measurement of exhaled lower respiratory nitric oxide and nasal nitric oxide. Am J Respir Crit Care Med 2005;171:912􀀁30.

21. ATS Statement: Guidelines for the Six-Minute Walk Test. Am J Respir Crit Care Med 2002;166:111-7.

22. Lammers AE, Hislop AA, Flynn Y, Haworth SG. The 6-minute walk test: normal values for children of 4-11 years of age. Arch Dis Child 2008;93:464-8.

23. American Thoracic Society. Standardisation of Spirometry. 1994 Update. Am J Respir Crit Care Med 1995;152:1107􀀁36.

24. Polgar G, Promadhat V. Pulmonary function testing in children: techniques and standards. In: Polgar G, Promadhat V, eds. Philadelphia: WB Saunders; 1971.

25. Goldenberg RL, Hauth JC, Andrews WW. Intrauterine infection and preterm delivery. N Engl J Med 2000;18;342:1500􀀁7.

26. Yoon BH, CAng JW, Romero R. Isolation of Ureaplasma urealyticum from the amniotic cavity and adverse outcome in preterm labor. Obstet Gynecol 1998;92:77–82.

27. Kasper DC, Mechtler TP, Reischer GH, Witt A, Langgartner M, Pollak A, et al. The bacterial load of Ureaplasma parvum in amniotic fluid is correlated with an increased intrauterine inflammatory response. Diagn Microbiol Infect Dis 2010 Jun;67:117􀀁21.

28. Gale NW, Yancopoulos GD. Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular development. Genes Dev 1999;13:1055􀀁66.

29. Jakkula M, Le Cras TD, Gebb S Hirth KP, Tuder RM, Voelkel NF, Abman SH. Inhibition of angiogenesis decreases alveolarization in the developing rat lung. Am J Physiol Lung Cell Mol Physiol 2000;279:600􀀁6.

30. Le Cras TD, Markham NE, Tuder RM, Voelkel NF, Abman SH. Treatment of newborn rats with a VEGF receptor inhibitor causes pulmonary hypertension and abnormal lung structure. Am J Physiol Lung Cell Mol Physiol 2002;283:555􀀁62.

31. Kinsella JP, Cutter GR, Walsh WF, Gerstmann DR, Bose CL, Hart C, et al. Early inhaled nitric oxide therapy in premature newborns with respiratory failure. N Engl J Med 2006;355:354􀀁64.

32. Ballard RA, Truog WE, Cnaan A, Martin RJ, Ballard PL, Merrill JD, et al. Inhaled nitric oxide in preterm infants undergoing mecanical ven-tilation. N Engl J Med 2006;355:343􀀁53.

33. Greenberg JM, Thompson FY, Brooks SK, McCormick-Shannon K, Cameron JE. Mesenchymal expression of VEGF D and A defines vascular patterning in developing lung. Dev Dyn 2002;224:144􀀁53.

34. Yancopoulos GD, Davis S, Gale NW, Rudge JS, Wiegand SJ, Holash J. Vascular-specific growth factors and blood vessel formation. Nature 2000;407:242–8.

35. Martin D, Galisteo R, Gutkind JS. CXCL8/IL8 stimulates vascular endothelial growth factor (VEGF) expression and the autocrine activation of VEGFR2 in endothelial cells by activating NFkappaB through the CBM (Carma3/Bcl10/Malt1) complex. J Biol Chem 2009; 284:6038􀀁42.

36. Kristan S, Marc M, Kern I, Flezar M, Suskovic S, Kosnik M, et al. Airway angiogenesis in stable and exacerbated chronic obstructive pul-monary disease. Scand J Immunol 2011;75:109􀀁14.

Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,200 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Chemical Abstracts Service Source Index The CAS Source Index (CASSI) Search Tool is an online resource that can quickly identify or confirm journal titles and abbreviations for publications indexed by CAS since 1907, including serial and non-serial scientific and technical publications.

Index Copernicus The Index Copernicus International (ICI) Journals database’s is an international indexation database of scientific journals. It covered international scientific journals which divided into general information, contents of individual issues, detailed bibliography (references) sections for every publication, as well as full texts of publications in the form of attached files (optional). For now, there are more than 58,000 scientific journals registered at ICI.

Geneva Foundation for Medical Education and Research The Geneva Foundation for Medical Education and Research (GFMER) is a non-profit organization established in 2002 and it works in close collaboration with the World Health Organization (WHO). The overall objectives of the Foundation are to promote and develop health education and research programs.

Scopus: CiteScore 1.0 (2022) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Embase Embase (often styled EMBASE for Excerpta Medica dataBASE), produced by Elsevier, is a biomedical and pharmacological database of published literature designed to support information managers and pharmacovigilance in complying with the regulatory requirements of a licensed drug.

Submission Turnaround Time

Conferences

Top