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Original Research

Open Access

Effects of melatonin on orofacial pain relief by regulating mitochondrial function in cell viability of peripheral sensory neurons

  • Yingying You1
  • Xianping Yi2
  • Hongwen He3,4,*,
  • Fang Huang3,4,*,

1Department of Stomatology, The Fifth Affiliated Hospital, Sun Yat-sen University, 519000 Zhuhai, Guangdong, China

2Department of Pathology, The Fifth Affiliated Hospital, Sun Yat-sen University, 519000 Zhuhai, Guangdong, China

3Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, 510623 Guangzhou, Guangdong, China

4Guangdong Provincial Key Laboratory of Stomatology, 510080 Guangzhou, Guangdong, China

DOI: 10.22514/sv.2022.075 Vol.18,Issue 6,November 2022 pp.81-93

Submitted: 01 August 2022 Accepted: 28 September 2022

Published: 08 November 2022

*Corresponding Author(s): Hongwen He E-mail:
*Corresponding Author(s): Fang Huang E-mail:


Melatonin (MT) is involved in the pain regulation of peripheral neurons, which is relevant to cell viability. This study aimed to examine the cell proliferation, cell cycle, cell apoptosis and intracellular mitochondrial function of ND7/23 and PC-12 cells treated with different physiological concentrations of MT. Our results showed that MT at concentrations of 10−8, 10−10 and 10−12 M inhibited cell proliferation and promoted the apoptosis of two cell lines, with the most significant changes observed at a concentration of 10−12 M. Further, 10−12 M MT promoted mitochondrial respiratory electron transfer and increased mitochondrial function in ND7/23 and PC-12 cells through the non-membrane receptor pathway. Comparatively, 10−8 M MT enhanced the mitochondrial effects of ND7/23 cells but showed opposite effects in PC-12 cells. In summary, MT affected cell viability through the non-membrane receptor pathway in a concentration-dependent manner and might be associated with pain regulations.


Melatonin; ND7/23; Cell viability; Mitochondrial function

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Yingying You,Xianping Yi,Hongwen He,Fang Huang. Effects of melatonin on orofacial pain relief by regulating mitochondrial function in cell viability of peripheral sensory neurons. Signa Vitae. 2022. 18(6);81-93.


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