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Original Research

Open Access

Physalin A exerts neuroprotective effects: inhibition of OGD/R-induced cellular pyroptosis and inflammatory responses in nerve cells

  • Huyin Yang1,*,
  • Dai Liu1
  • Yanping Wang1

1Department of Neurosurgery, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, 223300 Huai’an, Jiangsu, China

DOI: 10.22514/sv.2023.111 Vol.19,Issue 6,November 2023 pp.168-174

Submitted: 05 September 2023 Accepted: 13 October 2023

Published: 08 November 2023

*Corresponding Author(s): Huyin Yang E-mail: yangwenxi20180617@126.com

Abstract

A prevalent cerebrovascular disease like the acute cerebral infarction is caused by cerebral thrombosis or arteriosclerosis that can result in ischemic necrosis of brain tissues. Treatment drugs of higher efficiency are required to be developed for acute cerebral infarction. Physalin A (PA) is a major compound from Physalis alkekengi L. and has pharmacological properties. However, the neuroprotective effects of PA are less reported and the mechanism remains unclear. The acute cerebral infarction cell model was constructed by treating PC12 cells with oxygen-glucose deprivation/reoxygenation (OGD/R). The impacts of PA on cell viability were determined by performing 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) releasing assays. Immunoblot and Enzyme-linked Immunosorbent assay (ELISA) were conducted to examine PA effects on inflammation. PA effects on pyroptosis were detected by performing immunoblot and immunostaining. Moreover, immunoblot was further conducted to confirm the mechanism. In this study, the survival and inhibition of the pyroptosis of OGD/R-induced PC12 cells were regulated by PA. Furthermore, PA suppressed the inflammation. PA inhibited mitogen-activated protein kinase (MAPK) pathway activation, and the activation of MAPK pathway reversed the neuroprotective effect of PA. Therefore, PA exerts neuroprotective effects by suppressing OGD/R-induced cellular pyroptosis and inflammatory responses in nerve cells.


Keywords

Acute cerebral infarction; Physalin A (PA); OGD/R; Pyroptosis; MAPK pathway


Cite and Share

Huyin Yang,Dai Liu,Yanping Wang. Physalin A exerts neuroprotective effects: inhibition of OGD/R-induced cellular pyroptosis and inflammatory responses in nerve cells. Signa Vitae. 2023. 19(6);168-174.

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