Article Data

  • Views 570
  • Dowloads 151

Original Research

Open Access

Purpurogallin ameliorates sevoflurane-induced cognitive impairment and hippocampal neuroinflammation in neonatal mice

  • Yu Yao1
  • Zhihong Li2
  • Jingqi Zhang3
  • Jinzhuo Wang4,*,
  • Baohua Wang1
  • Fengjuan Wang1

1Department of Anesthesiology, Affiliated Hospital of Changchun University of Chinese Medicine, 130021 Changchun, Jilin, China

2Obstetrics and Gynecology Diagnosis and Treatment Center, Affiliated Hospital of Changchun University of Chinese Medicine, 130021 Changchun, Jilin, China

3Department of Massage, Affiliated Hospital of Changchun University of Chinese Medicine, 130021 Changchun, Jilin, China

4Department of Anesthesiology, Changchun Obstetrics-Gynecology Hospital, 130000 Changchun, Jilin, China

DOI: 10.22514/sv.2024.032 Vol.20,Issue 3,March 2024 pp.89-96

Submitted: 02 November 2023 Accepted: 23 January 2024

Published: 08 March 2024

*Corresponding Author(s): Jinzhuo Wang E-mail: wjzh27@126.com

Abstract

Postoperative cognitive dysfunction contributes to memory impairment, which is involved in neurotoxicity, endoplasmic reticulum stress, calcium overload and hippocampal neuron apoptosis. Purpurogallin can mitigate ischemia-induced neuronal injury and microglial inflammation. However, whether purpurogallin affects cognitive dysfunction remains unclear. To investigate the efficacy of purpurogallin on cognitive dysfunction and explore the potential signal pathways, Sevoflurane (SEV)-induced model was established on mice, and water maze testing was performed to record escape latency, platform residence time, platform crossings and swimming speed analysis. The concentration of Interleukin (IL)-6, IL-1β and Tumor necrosis factor (TNF)-α in hippocampus tissue were evaluated using Enzyme-linked immunosorbent assay (ELISA). The hippocampal neurons were analyzed using Nissl staining. The Iba-1 was evaluated using immunofluorescent assay (IFA), while the cell apoptosis was assessed using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Western blots were performed to evaluate the expression of Ionized calcium binding adaptor molecule 1 (Iba1), integrin α-M (CD11b), Bax, integrin α-M (Bcl-2), p-65 and IκBα, as well as the phosphorylation of p-65 and IκBα. Purpurogallin at dose of 150 mg/kg significantly reduced escape latency in SEV-induced mice (p < 0.001), without affecting swimming speed. It increased time spent in the target quadrant and restored platform crossings reduced by SEV. Purpurogallin also mitigated the elevated IL-6, IL-1β and Transforming growth factor alpha (TGF-α) levels caused by SEV in the hippocampus, preserved Nissl bodies, and reduced Iba-I and CD11b expression (p < 0.01). It alleviated cell apoptosis by downregulating Bax and upregulating Bcl-2, while inhibiting p-65 phosphorylation and promoting IκBα phosphorylation induced by SEV (p < 0.05). Purpurogallin effectively countered SEV-induced cognitive impairment, neuroinflammation, hippocampal injury, microglial activation and neuronal apoptosis through inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway, which facilitate purpurogallin to become a drug candidate for postoperative cognitive dysfunction.


Keywords

Cognitive dysfunction; Microglial activation; Neuronal apoptosis; NF-κB pathway; Neuroinflammation; Purpurogallin


Cite and Share

Yu Yao,Zhihong Li,Jingqi Zhang,Jinzhuo Wang,Baohua Wang,Fengjuan Wang. Purpurogallin ameliorates sevoflurane-induced cognitive impairment and hippocampal neuroinflammation in neonatal mice. Signa Vitae. 2024. 20(3);89-96.

References

[1] Yang Y, Liu Y, Zhu J, Song S, Huang Y, Zhang W, et al. Neuroinflammation-mediated mitochondrial dysregulation involved in postoperative cognitive dysfunction. Free Radical Biology and Medicine. 2022; 178: 134–146.

[2] Zhao G, Jing J. HOMER1A restores sevoflurane-induced cognitive dysfunction by regulating microglia’s activation through activating the AMPK/TXNIP axis. Signa Vitae. 2023; 19: 202–212.

[3] Wang P, Wang P, Yin M, Wang S. Sevoflurane ameliorates LPS-induced inflammatory injury of HK-2 cells through Sirtuin1/NF-κB pathway. Allergologia et Immunopathologia. 2022; 50: 115–123.

[4] Zhang Q, Li Y, Yu J, Yin C, Guo J, Zhao J, et al. TLR3 deletion inhibits programmed necrosis of brain cells in neonatal mice with sevoflurane-induced cognitive dysfunction. Aging. 2022; 14: 4714–4727.

[5] Kim K, Kim TH, Ihn HJ, Kim JE, Choi J-Y, Shin H-I, et al. Inhibitory effect of purpurogallin on osteoclast differentiation in vitro through the downregulation of c-Fos and NFATc1. International Journal of Molecular Sciences. 2018; 19: 601.

[6] Park HY, Kim TH, Kim CG, Kim GY, Kim CM, Kim ND, et al. Purpurogallin exerts anti-inflammatory effects in lipopolysaccharide-stimulated BV2 microglial cells through the inactivation of the NF-κB and MAPK signaling pathways. International Journal of Molecular Medicine. 2013; 32: 1171–1178.

[7] Cheng Z, Li X, Ye X, Yu R, Deng Y. Purpurogallin reverses neuronal apoptosis and enhances “M2” polarization of microglia under ischemia via mediating the miR-124-3p/TRAF6/NF-κB Axis. Neurochemical Research. 2023; 48: 375–392.

[8] Liu L, Huan L, Zhang Y, Wei W, Chen Z, Xu D, et al. Ubiquitin-specific protease 8 inhibits lipopolysaccharide-triggered pyroptosis of human bronchial epithelial cells by regulating PI3K/AKT and NF-κB pathways. Allergologia et Immunopathologia. 2022; 50: 96–103.

[9] Zhao W, Song S, Chu W, Li Y, Chen S, Ji Y, et al. Disruption of hippocampal P2RX2/CaMKII/NF-κB signaling contributes to learning and memory impairment in C57BL/6 mice induced by surgery plus anesthesia in neonatal period. Biomedicine & Pharmacotherapy. 2022; 149: 112897.

[10] Sun M, Dong Y, Li M, Zhang Y, Liang F, Zhang J, et al. Dexmedetomidine and clonidine attenuate sevoflurane-induced tau phosphorylation and cognitive impairment in young mice via α-2 adrenergic receptor. Anesthesia and Analgesia. 2021; 132: 878–889.

[11] Li X, Cheng Z, Chen X, Yang D, Li H, Deng Y. Purpurogallin improves neurological functions of cerebral ischemia and reperfusion mice by inhibiting endoplasmic reticulum stress and neuroinflammation. International Immunopharmacology. 2022; 111: 109057.

[12] Jaszczyk A, Stankiewicz AM, Juszczak GR. Dissection of mouse hippocampus with its dorsal, intermediate and ventral subdivisions combined with molecular validation. Brain Sciences. 2022; 12: 799.

[13] Huang J, Dai W, Xiao D, Xiong Q, Liu C, Hu J, et al. Acetylation-dependent SAGA complex dimerization promotes nucleosome acetylation and gene transcription. Nature Structural & Molecular Biology. 2022; 29: 261–273.

[14] Liu X, Nasab EM, Athari SS. Anti-inflammatory effect of N-(trifluoromethylphenyl)-2-cyano-3-hydroxy-crotonic acid amide and gluconic acid on allergic rhinitis and asthma controlling. Allergologia et Immunopathologia. 2022; 50: 71–75.

[15] Chen J, Zheng ZV, Lu G, Chan WY, Zhang Y, Wong GKC. Microglia activation, classification and microglia-mediated neuroinflammatory modulators in subarachnoid hemorrhage. Neural Regeneration Research. 2022; 17: 1404–1411.

[16] Lu J, Liu Z, He W, Shao S. Knockdown of TNFAIP1 mitigates sevoflurane-induced cognitive dysfunction by activating CREB/Nrf2 pathway. Tropical Journal of Pharmaceutical Research. 2022; 21: 1419–1424.

[17] Jouybar R, Kazemifar S, Asmarian N, Karami A, Khademi S. Comparison of the effect of melatonin, dexmedetomidine, and gabapentin on reduction of postoperative pain and anxiety following laminectomy: a randomized clinical trial. BMC Anesthesiology. 2022; 22: 318.

[18] Karami F, Jamaati H, Coleman-Fuller N, Zeini MS, Hayes AW, Gholami M, et al. Is metformin neuroprotective against diabetes mellitus-induced neurodegeneration? An updated graphical review of molecular basis. Pharmacological Reports. 2023; 75: 511–543.

[19] Guo Y, Chen Q, Wu B, Sun L. Isovitexin restores sevoflurane-induced cognitive dysfunction by mediating autophagy through activation of the PGC-1α/FNDC5 signaling pathway. Acta Neurobiologiae Experimentalis. 2022; 82: 373–379.

[20] Zhen AX, Piao MJ, Hyun YJ, Kang KA, Ryu YS, Cho SJ, et al. Purpurogallin protects keratinocytes from damage and apoptosis induced by ultraviolet b radiation and particulate matter 2.5. Biomolecules & Therapeutics. 2019; 27: 395–403.

[21] Islam F, Islam MM, Khan Meem AF, Nafady MH, Islam MR, Akter A, et al. Multifaceted role of polyphenols in the treatment and management of neurodegenerative diseases. Chemosphere. 2022; 307: 136020.

[22] Xie X, Zu X, Liu F, Wang T, Wang X, Chen H, et al. Purpurogallin is a novel mitogen-activated protein kinase kinase 1/2 inhibitor that suppresses esophageal squamous cell carcinoma growth in vitro and in vivo. Molecular Carcinogenesis. 2019; 58: 1248–1259.


Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,200 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Chemical Abstracts Service Source Index The CAS Source Index (CASSI) Search Tool is an online resource that can quickly identify or confirm journal titles and abbreviations for publications indexed by CAS since 1907, including serial and non-serial scientific and technical publications.

Index Copernicus The Index Copernicus International (ICI) Journals database’s is an international indexation database of scientific journals. It covered international scientific journals which divided into general information, contents of individual issues, detailed bibliography (references) sections for every publication, as well as full texts of publications in the form of attached files (optional). For now, there are more than 58,000 scientific journals registered at ICI.

Geneva Foundation for Medical Education and Research The Geneva Foundation for Medical Education and Research (GFMER) is a non-profit organization established in 2002 and it works in close collaboration with the World Health Organization (WHO). The overall objectives of the Foundation are to promote and develop health education and research programs.

Scopus: CiteScore 1.0 (2022) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Embase Embase (often styled EMBASE for Excerpta Medica dataBASE), produced by Elsevier, is a biomedical and pharmacological database of published literature designed to support information managers and pharmacovigilance in complying with the regulatory requirements of a licensed drug.

Submission Turnaround Time

Conferences

Top