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Original Research

Open Access

Moscatilin inhibits sevoflurane-induced inflammatory and oxidative stress injury in hippocampal neuronal HT22 cells

  • Lulu Zhang1,*,†,
  • Yuhang Zhang2,†

1Department of Anesthesiology, Zigong Fourth People’s Hospital, 643000 Zigong, Sichuan, China

2Graduate School, Chongqing Medical University, 400016 Chongqing, China

DOI: 10.22514/sv.2024.073 Vol.20,Issue 6,June 2024 pp.67-73

Submitted: 20 February 2024 Accepted: 17 April 2024

Published: 08 June 2024

*Corresponding Author(s): Lulu Zhang E-mail:

† These authors contributed equally.


In order to analyze the effect and mechanism of Moscatilin on sevoflurane-induced HT22 hippocampal neuron cell (HT22) injury, sevoflurane was applied to HT22 cells to create an in vitro model of nerve damage, and Moscatilin was given to observe its effect on indicators of nerve injury. The effect of Moscatilin on sevoflurane-induced cell injury was measured based on cell viability (Cell Counting Kit-8), the expression of inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the cells was detected by Enzyme-Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR). Reactive oxygen species (ROS) production in the neuronal cells was detected by oxidative stress measurements based on the 2′,7′-Dichlorofluorescein (DCF) assay. Using flow cytometry, the rate of apoptosis was determined, and the expression of apoptosis-related markers cleaved-caspase3 and cleaved-Poly Adp Ribose Polymerase (cleaved-PARP), and nuclear factor-k-gene binding (NF-κB)-related proteins were detected by western-blot analysis. The results showed that sevoflurane caused a significant decrease in HT22 cell viability, an increase in inflammatory factors TNF-α, IL-6 and IL-1β, ROS and apoptosis, an increase in apoptosis-related markers, cleaved-caspase3 and cleaved-PARP, as well as in the p-p65/p65 ratio of NF-κB-related proteins, and a decrease in the expression of inhibitor of NF-κBα (IκBα) protein. Moscatilin treatment counteracted sevoflurane-induced reduction in cell viability, decreased inflammatory factors and ROS production, inhibited sevoflurane-induced apoptosis, suppressed p-p65/p65 ratio, and increased IκBα protein expression. In conclusion, Moscatilin inhibits the NF-κB pathway and ameliorates sevoflurane-induced inflammatory responses, ROS production and apoptosis in HT22 hippocampal neuronal cells.


Sevoflurane; Moscatilin; NF-κB; ROS; Apoptosis

Cite and Share

Lulu Zhang,Yuhang Zhang. Moscatilin inhibits sevoflurane-induced inflammatory and oxidative stress injury in hippocampal neuronal HT22 cells. SignaVitae. 2024. 20(6);67-73.


[1] Xia H, Li Y, Zhu G, Zhang X. Activation of mitochondria apoptotic pathway is involved in the sevoflurane-induced hippocampal neuronal HT22 cells toxicity through miR-145/Binp3 axis. International Journal of Clinical and Experimental Pathology. 2017; 10: 10873–10882.

[2] Sun L, Hong X, Li Y, Wang D. SESN1, as a potential target for postoperative cognitive dysfunction, attenuates sevoflurane-induced neuronal cell damage in the hippocampus. Signa Vitae. 2023; 19: 137–143.

[3] Yuan Z, Zhu M, Wei X, Li X. Eriocitrin alleviates sevoflurane-induced cytotoxicity in HT22 cells via Nrf2 pathway. Tropical Journal of Pharmaceutical Research. 2022; 21: 259–264.

[4] Zhang H, Yan L. Solasonine relieves sevoflurane-induced neurotoxicity via activating the AMP-activated protein kinase/FoxO3a pathway. Human & Experimental Toxicology. 2022; 41: 09603271211069984.

[5] Liu YN, Pan SL, Peng CY, Huang DY, Guh JH, Chen CC, et al. Moscatilin repressed lipopolysaccharide-induced HIF-1alpha accumulation and NF-kB activation in murine RAW264.7 cells. Shock. 2010; 33: 70–75.

[6] Su W, Zeng L, Chen W. Moscatilin suppresses the breast cancer both in vitro and in vivo by inhibiting HDAC3. Dose-Response. 2021; 19: 15593258211001251.

[7] Chen WK, Chen CA, Chi CW, Li LH, Lin CP, Shieh HR, et al. Moscatilin inhibits growth of human esophageal cancer xenograft and sensitizes cancer cells to radiotherapy. Journal of Clinical Medicine. 2019; 8: 187.

[8] Huang J, Huang F, Yang C. Moscatilin ameliorates tau phosphorylation and cognitive deficits in Alzheimer’s disease models. Journal of Natural Products. 2019; 82: 1979–1988.

[9] Yang Z, Yuan C. IL-17a promotes the neuroinflammation and cognitive function in sevoflurane anesthetized aged rats via activation of NF-κB signaling pathway. BMC Anesthesiology. 2018; 18: 147.

[10] Manríquez-Olmos L, Garrocho-Rangel A, Pozos-Guillén A, Ortiz-Magdaleno M, Escobar-García DM. Effect of tricalcium silicate cements in gene expression of COL1A1, MAPK’s, and NF-kB, and cell adhesion in primary teeth’ pulp fibroblasts. Journal of Clinical Pediatric Dentistry. 2022; 46: 17–24.

[11] Tang D, Xi R, Zeng X. Interaction of Pten gene and AKT/mTOR pathway in endometrial adenocarcinoma proliferation. European Journal of Gynaecological Oncology. 2022; 43: 265–270.

[12] Liang M, Wang F, Li Q, Lei X, Chen Y, Hu N. LncRNA SNHG3 promotes sevoflurane-induced neuronal injury by activating NLRP3 via NEK7. Neurochemical Research. 2023; 48: 2754–2766.

[13] Chen X, Li C, Zeng R, Qiu L, Huang J, Wang N, et al. Inhibition of miR-495-3p ameliorated sevoflurane induced damage through BDNF/ERK/CREB signaling pathways in HT22 cells. Transplant Immunology. 2022; 75: 101708.

[14] Lai MC, Liu WY, Liou S, Liu I. The protective effects of Moscatilin against methylglyoxal-induced neurotoxicity via the regulation of p38/JNK MAPK pathways in PC12 neuron-like cells. Food and Chemical Toxicology. 2020; 140: 111369.

[15] Huang H, Hu C, Xu L, Zhu X, Zhao L, Min J. The effects of hesperidin on neuronal apoptosis and cognitive impairment in the sevoflurane anesthetized rat are mediated through the PI3/Akt/PTEN and nuclear factor-κB (NF-κB) signaling pathways. Medical Science Monitor. 2020; 26: e920522.

[16] Tang XL, Wang X, Fang G, Zhao YL, Yan J, Zhou Z, et al. Resveratrol ameliorates sevoflurane-induced cognitive impairment by activating the SIRT1/NF-κB pathway in neonatal mice. The Journal of Nutritional Biochemistry. 2021; 90: 108579.

[17] Li Q, Zhang X, Li S, Li W, Teng Y, Zhou Y, et al. Carnosol alleviates sevoflurane‑induced cognitive dysfunction by mediating NF-κB pathway in aged rats. Drug Development Research. 2022; 83: 1342–1350.

[18] Lai MC, Liu WY, Liou SS, Liu IM. A bibenzyl component Moscatilin mitigates glycation-mediated damages in an SH-SY5Y cell model of neurodegenerative diseases through AMPK activation and RAGE/NF-κB pathway suppression. Molecules. 2020; 25: 4574.

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