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Original Research

Open Access

In vitro effects of Ganoderic acid A on NF-κB-mediated inflammatory response in caerulein-stimulated pancreatic acinar cells

  • Yuzhou Jin1
  • Jin Huang1,*,
  • Na Ma2,*,

1Department of Gastroenterology, The Affiliated Changzhou No.2 People’s Hospital with Nanjing Medical University, 213161 Changzhou, Jiangsu, China

2Department of Emergency, The Affiliated Changzhou No.2 People’s Hospital with Nanjing Medical University, 213161 Changzhou, Jiangsu, China

DOI: 10.22514/sv.2024.075 Vol.20,Issue 6,June 2024 pp.93-98

Submitted: 26 February 2024 Accepted: 13 May 2024

Published: 08 June 2024

*Corresponding Author(s): Jin Huang E-mail: jin_huang0713@163.com
*Corresponding Author(s): Na Ma E-mail: mn715292364@sina.com

Abstract

This study aimed to investigate Ganoderic acid A’s (GAA) possible effects on inflammation and oxidative stress of caerulein-stimulated pancreatic acinar cells and uncover its effects on acute pancreatitis (AP). A cell model of AP was constructed by treating pancreatic acinar AR42J cells with caerulein. Cell counting kit-8 (CCK-8) assay was used to measure cell viability, while Enzyme-Linked Immunosorbent Assay (ELISA) and 2′,7′-Dichlorofluorescein (DCF) fluorescence assays were used to measure inflammation and oxidative stress. Western blot analysis was used to investigate Nuclear Factor-kappa B (NF-κB) signaling pathway inhibition, focusing on p65 and NF-kappa-B inhibitor alpha (IκBα) phosphorylation states. GAA significantly enhanced cell viability in caerulein-stimulated pancreatic acinar AR42J cells. It also significantly reduced AR42J cells’ inflammatory response. Furthermore, GAA treatment mitigated oxidative stress by decreasing Reactive oxygen species (ROS) production. Lastly, GAA inhibited the NF-κB pathway, as evidenced by decreased p65 and IκBα phosphorylation. By inhibiting NF-B-mediated inflammation, GAA attenuated caerulein-induced AP.


Keywords

Acute pancreatitis (AP); Ganoderic acid A (GAA); Inflammatory response; Oxidative stress; NF-κB pathway


Cite and Share

Yuzhou Jin,Jin Huang,Na Ma. In vitro effects of Ganoderic acid A on NF-κB-mediated inflammatory response in caerulein-stimulated pancreatic acinar cells. SignaVitae. 2024. 20(6);93-98.

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