The effect of Daucus carota L. var atrorubens on the treatment of experimental sepsis models

Background: Sepsis secondary to infections is a critical health issue with limited preventive treatments. Various studies have shown that Daucus carota L. ssp. Atrorubens (black carrot) possesses antibacterial, antifungal, anti-inflammatory, antiseptic and hepatoprotective effects. This study investigated the therapeutic potential of black carrot, focusing on its anti-inflammatory and antioxidant effects in an experimental sepsis model. Methods: Thirty two Wistar Albino rats were divided equally into four groups. Group I served as the control without any treatment. The caecal ligation and perforation (CLP) procedure was performed on Groups II, III and IV. Group II underwent only the CLP procedure. Group III received 3 mL intraperitoneal isotonic sodium chloride, and Group IV was administered 3 mL black carrot solution every 24 h for 7 days post-CLP. On day 7, all rats were sacrificed, and blood, kidney, and liver samples were collected for analysis. Data were evaluated using GraphPad Prism 6.0 software and analysed by one-way analysis of variance and Tukey multiple comparison tests. Results: Biochemical analysis showed similar Aspartate Transaminase (AST) and Alanine Aminotransferase (ALT) values between the control and black carrot groups. Histopathological examination revealed minimal polymorphonuclear leukocyte infiltration in the CLP + black carrot group, and normal-appearing arteria hepaticus interlobularis, vena interlobularis and ductus biliferi, similar to the control group. Despite no significant decrease in serum urea levels, histopathological findings indicated a nephroprotective effect. The CLP + black carrot group showed reduced tubular dilatation and brush border loss and appeared similar to the control group. Conclusions: Overall, black carrot showedhepatoprotective, nephroprotective, anti-inflammatory and antioxidant effects, as supported by both biochemical and histological data. These findings suggest that black carrot may offer therapeutic benefits in managing sepsis-induced organ damage.

Daucus carota; Sepsis; Hepatoprotective; Black carrot; Nephroprotective

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