Sodium aesculin alleviates inflammation and pyroptosis in cardiomyocytes induced by methyl parathion in vivo and in vitro AOPP model

Background: Acute organophosphorus pesticide poisoning (AOPP) is a prevalent cause of drug-related poisoning in hospital emergency departments, yet the mechanisms underlying myocardial damage remain poorly understood. Sodium aesculin, the active compound found in Aesculin, has demonstrated promising anti-inflammatory and antioxidant properties. However, its effects on myocardial injury induced by AOPP have not been thoroughly investigated. This study aims to explore the potential protective effects of sodium aesculin (SA) on methyl parathion (MP)-induced cardiomyocyte injury. Methods: MP-induced cardiomyocytes were utilized as an experimental cell model. Cell viability was assessed using Cell Counting Kit-8 (CCK-8) assay, which provided insights into the compound’s effects on cellular proliferation. Quantitative Polymerase Chain Reaction (qPCR) was employed to evaluate the impact of Sodium aesculin on inflammatory responses and pyroptotic cell death within both cellular and rat models. Immunoblot assays were further conducted to confirm the mechanism. Results: Using CCK-8, qPCR and immunoblot assays in H9C2 cells and rats, we observed that SA increased MP-impaired cell viability (p < 0.01). A dosage of 1.5 mg/kg SA reduced cardiac inflammation (p < 0.001) and pyroptosis (p < 0.001) compared to MP controls. Mechanistically, sodium aesculin inhibits MP-induced Nuclear Factor Kappa-Light-Chain-Enhancer of Activated B Cells (NF-κB) activation (p < 0.001). Conclusions: Sodium aesculin (SA) alleviates inflammation and pyroptosis in MP-induced cardiomyocytes, suggesting its potential as a therapeutic agent for AOPP-related myocardial injury.

Acute organophosphorus pesticide poisoning (AOPP); Cardiomyocyte; Inflammation; Pyroptosis; NF-κB

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